Identification of 3'-terminal exons from yeast artificial chromosomes.
نویسندگان
چکیده
منابع مشابه
Identification of 3'-terminal exons from yeast artificial chromosomes.
We report an extension of 3'-terminal exon trapping technology to the identification of transcribed sequences from yeast artificial chromosomes (YACs). A 350-kb YAC containing mouse genomic DNA was gel-purified and used as the target DNA for the 3'-terminal exon trapping strategy. A novel direct ligation/transfection approach was employed to increase the efficiency of trapping 3'-terminal exons...
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Editorial Board Rakesh Anand Zeneca Pharmaceuticals Johannes Bos University of Utrecht Anne Bowcock University of Texas Southwestern Medical Center Jeff Chamberlain University of Michigan Medical School Nicholas Dracopoli National Center for Human Genome Research Joe Ecker University of Pennsylvania Ray Fenwick Dianon Systems, Inc. Kenshi Hayashi National Cancer Center Research Institute, Tokyo...
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A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the beta-galactosidase gene as an expression reporter in mammalian cells.
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ژورنال
عنوان ژورنال: Genome Research
سال: 1995
ISSN: 1088-9051
DOI: 10.1101/gr.4.6.322